Fig. 6

PTEN gene knockdown enhanced the protein expression of α/γ-ENaC in AT2 cells. a, c Representative Western blot bands of PTEN and α/γ-ENaC protein expression in LPS-treated AT2 cells transfected with miR-130b mimic/inhibitor or siPTEN. b, d and e Graphical representation of data obtained from Western blot assays for PTEN and α/γ-ENaC. Bands were quantified using gray analysis (PTEN/β-actin, α-ENaC/β-actin and γ-ENaC/β-actin). **P < 0.01, compared with control; ^&P < 0.05, compared with LPS + miR-130b mimic negative control (NC); ^#P < 0.05, compared with LPS + miR-130b inhibitor negative control (Inhibitor NC); ^$P < 0.05, compared with LPS + siPTEN, n = 4–6