Fig. 5

MiR-130b directly targets PTEN and BMSCs transfected with miR-130b mimic/miR-130b inhibitor affect protein expressions of PTEN and α/γ-ENaC in AT2 cells. a The potential binding sites for miR-130b on the 3′-UTRs of PTEN. b Dual luciferase assay for miR-130b binding with PTEN in H441 cells. H441 cells were co-transfected with miR-130b mimic negative control (NC) or miR-130b mimic (Mimic) together with pmirGLO-PTEN (WT or MT) for 24 h. c, e Representative Western blot bands of PTEN and α/γ-ENaC protein expression in AT2 cells co-cultured with BMSCs that were transfected with miR-130b mimic/inhibitor. d, f and g Graphical representation of data obtained from Western blot assays for PTEN and α/γ-ENaC. Bands were quantified using gray analysis (PTEN/β-actin, α-ENaC/β-actin and γ-ENaC/β-actin). *P < 0.05, **P < 0.01, compared with miR-130b mimic negative control or plus wild-type pmirGLO-PTEN (NC or NC + WT); ^&P < 0.05, compared with miR-130b inhibitor negative control (Inhibitor NC), n = 4