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Fig. 1 | Respiratory Research

Fig. 1

From: Bone marrow mesenchymal stem cells derived miRNA-130b enhances epithelial sodium channel by targeting PTEN

Fig. 1

LPS treatment enhances IL-8 levels and BMSCs increase protein and transcription level of ENaC in AT2 cells. a, b IL-8 levels were measured by ELISA kit after LPS treatment of different dosage and time in AT2 cells. c Representative Western blot bands of α- and γ-ENaC protein expression in AT2 cells treated with LPS for 12 h and/or co-cultured with BMSCs for 24 h. Blots for β-actin were used as internal controls. d, e Graphical representation of data obtained from Western blot and quantified through gray analysis (α- or γ-ENaC/β-actin). f–h qRT-PCR results for ENaC mRNAs. Relative level of ENaC mRNA were calculated as α-, β- or γ-ENaC/GAPDH ratios. *P < 0.05, **P < 0.01, compared with control; &P < 0.05, compared with LPS, n = 5–6

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