Fig. 3

FFAR1 agonists attenuate carbachol-induced MLC phosphorylation in HASM cells. HASM cells were pre-treated with vehicle (DMSO), GW9508 (0.1–10 µM), TAK875 (10 µM), or Formoterol (10 nM) for 10–30 min, then stimulated with carbachol for 10 min. Agonists-induced MLC phosphorylation were determined. a, b 10 µM GW9508 pre-treatment for 30 min decreased CCh-induced MLC phosphorylation. (n = 5 donors) c, d 10 µM TAK875 pre-treatment for decreased CCh-induced MLC phosphorylation. (n = 7 donors) e, f 10 µM GW9508 and TAK875 pre-treatment for 30 min has little effect on CCh-induced MYPT1 phosphorylation. g, h TAK875 attenuated CCh-induced MLC phosphorylation to a comparable magnitude in non-obese and obese donor derived HASM cells (n = 3–5 donors). (One-way ANOVA with Dunnett’s Test, compared to Veh; ns not significant; p < 0.05: *, p < 0.01: **, p < 0.001: ***)