Fig. 2

FFAR1 agonist TAK875 attenuates histamine-induced MLC phosphorylation in HASM cells. HASM cells were pre-treated with vehicle (DMSO), GW9508 (0.1–10 µM), TAK875 (10 µM), or Formoterol (10 nM) for 10–30 min, then stimulated with histamine 2.5 µM for 10 min. Agonists-induced MLC phosphorylation were determined. a, b 10 µM TAK875 pre-treatment for 30 min decreased histamine-induced MLC phosphorylation. (n = 7 donors) c, d 10 µM GW9508 or TAK875 pre-treatment has little effect on histamine-induced [Ca²⁺]_i. (n = 6 donors) (One-way ANOVA with Dunnett’s test, compared to DMSO; ns not significant; p < 0.01: **, p < 0.001: ***)