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Fig. 1 | Respiratory Research

Fig. 1

From: FFAR1 activation attenuates histamine-induced myosin light chain phosphorylation and cortical tension development in human airway smooth muscle cells

Fig. 1

FFAR1 agonist reduced histamine-induced cell stiffness in HASM cells. HASM cells were derived from 2 lung donors. To control for random effects due to multiple cell measurements from the same donor, we applied mixed effect model using SAS V.9.2 (SAS Institute Inc., Cary, NC). HASM cells were pre-treated with vehicle (DMSO), GW9508 (10 µM), or TAK875 (10 µM) for 30 min. Following 30 min pretreatments, cell stiffness was measured for 60 s, and after histamine (2.5 µM) addition (t = 60 s), stiffness was continuously measured for the next 240 s. Data are presented as Estimated Mean ± SE, n = 267–278 cell measurements per treatments from 2 donors; p < 0.05: *)

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