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Fig. 6 | Respiratory Research

Fig. 6

From: GITRL on dendritic cells aggravates house dust mite-induced airway inflammation and airway hyperresponsiveness by modulating CD4+ T cell differentiation

Fig. 6

Knockdown of GITRL on BMDCs partially restored the balance of Th1/Th2 and Th17/Treg cells. a Expression of GFP and the morphologic characteristics of BMDCs were observed under a fluorescence microscope (× 100 magnification, scale bars = 100 μm). b Flow cytometry analysis of the percentage of GFP+ cells in BMDCs. c Representative western blots of GITRL protein in LV-GFP-, LV-shGITRL-1-, LV-shGITRL-2-, and LV-shGITRL-3-transduced BMDCs. d Quantification of western blot data from (c) (n = 4—5 per group). e GITRL mRNA expression in each group (n = 6—7 per group). f Immunofluorescent staining for GITRL in HDM-treated LV-GFP and LV-shGITRL BMDCs (× 200 magnification, scale bars = 50 μm). g Quantification of the GITRL mean fluorescent intensity (MFI) in HDM treated LV-GFP and LV-shGITRL BMDCs (n = 6 per group). h Flow cytometry analysis of the percentage of CD4+CD25+Foxp3+ T cells (Tregs) in splenocytes cocultured with BMDCs. i Statistical analysis of (h) (n = 6 per group). j Flow cytometry analysis of the percentage of CD4+IL-4+ T cells (Th2), CD4+IFN-γ+ T cells (Th1) and CD4+IL-17+ T cells (Th17) in splenocytes cocultured with BMDCs. km Statistical analysis of (j) (n = 6 per group). nq Foxp3, GATA3, RORγt and T-bet mRNA expression in each group (n = 6 per group). *P < 0.05, **P < 0.01, ***P < 0.001

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