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Fig. 5 | Respiratory Research

Fig. 5

From: GITRL on dendritic cells aggravates house dust mite-induced airway inflammation and airway hyperresponsiveness by modulating CD4+ T cell differentiation

Fig. 5

GITRL is increased on mouse bone marrow-derived dendritic cells stimulated by HDM in vitro. a Flow cytometry analysis of the percentage of CD11c+ cells. b Representative western blots of GITRL protein in PBS or HDM-treated BMDCs. c Quantification of western blots data from (b) (n = 4–5 per group). d Immunofluorescent staining for GITRL in PBS or HDM-treated BMDCs (× 200 magnification, scale bars = 50 μm). e Quantification of the GITRL mean fluorescent intensity (MFI) in PBS- or HDM-treated BMDCs (n = 6 per group). f Flow cytometry analysis of the percentage of CD11c+MHCII+GITRL+ cells in PBS- or HDM-treated BMDCs. g Statistical analysis of (f) (n = 4–5 per group). *P < 0.05, ***P < 0.001

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