Table 1 The sequence of primers
Name | Sequence |
|---|---|
Sequences of synthesized RNA nucleotides | |
siR-MALAT1-forward | 5′-GGAGUACCCUGAAGCUAUAUU-3′ |
siR-MALAT1-reverse | 5′-UAUAGCUUCAGGGUACUCCUU-3′ |
siR-NC-forward | 5′-UUCUCCGAACGUGUCACGUUU-3′ |
siR-NC-reverse | 5′-ACGUGACACGUUCGGAGAAUU-3′ |
mimic miR-181a-5p-forward | 5′-AACAUUCAACGCUGUCGGUGAGU-3′ |
mimic miR-181a-5p-reverse | 5′-UUUUGUAAGUUGCGACAGCCACU-3′ |
mimic NC-forward | 5′-UUCUCCGAACGUGUCACGUTT-3′ |
mimic NC-reverse | 5′-TTAAGAGGCUUGCACAGUGCA-3′ |
Primers used in qRT-PCR | |
MALAT1-forward | 5′-GCTCTGTGGTGTGGGATTGA-3′ |
MALAT1-reverse | 5′-GTGGCAAAATGGCGGACTTT-3′ |
miR-181a-5p-forward | 5′-ACACTCCAGCTGGGAACATTCAACGCTGTCGG-3' |
miR-181a-5p-reverse | 5′-TGGTGTCGTGGAGTCG-3′ |
Fas-forward | 5′-CTGCATCATGATGGCCAATTCTGC-3′ |
Fas-reverse | 5′-ATGACACTAAGTCAAGTTAAAGGC-3′ |
TNF-α-forward | 5′-GCTGCACTTTGGAGTGATCG-3′ |
TNF-α-reverse | 5′-TCACTCGGGGTTCGAGAAGA-3′ |
IL‐1β-forward | 5′- CTGAGCTCGCCAGTGAAATG-3′ |
IL‐1β-reverse | 5′-TGTCCATGGCCACAACAACT-3′ |
IL‐6-forward | 5′-TTCTACAGACTACGGTTTGAG-3′ |
IL‐6-reverse | 5′-GGATGACACAGTGATGCT-3′ |
U6-forward | 5′-GCTTCGGCAGCACATATACTAAAAT-3′ |
U6-reverse | 5′-CGCTTCACGAATTTGCGTGTCAT-3′ |
β-actin-forward | 5′-AGAAAATCTGGCACCACACC-3′ |
β-actin-reverse | 5′-CCATCTCTTGCTCGAAGTCC-3′ |