Fig. 2

miRNA-181a-5p inhibits LPS-induced HPMEC apoptosis through directly targeting Fas. Mimic miR-181a-5p or mimic miR-NC was transfected to HPMECs, which were then treated with LPS. a Increased miR-181a-5p expression by mimic miR-181a-5p. b Decreased LPS-induced Fas mRNA level by mimic miR-181a-5p. c Decreased LPS-induced Fas protein level by mimic miR-181a-5p. d Decreased LPS-induced HPMEC apoptosis by mimic-miR-181a-5p. e pGL3-FAS-WT or pGL3-FAS-MUT plasmids were co-transfected with mimic miR-181a-5p or miR-NC. f Luciferase assay revealed that mimic miR-181a-5p decreased the luciferase activity in pGL3-FAS-WT but not in pGL3-FAS-MUT. *, **p < 0.05, 0.01.