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Table 3 RNA integrity of sputum samples supplied to gene expression analysis

From: Exploration of the sputum methylome and omics deconvolution by quadratic programming in molecular profiling of asthma and COPD: the road to sputum omics 2.0

 

RIN

Preservation

Mean ± SD (min/max)

(RLT/HOPE)

Asthma

n = 9

6.4 ± 2.5 (3.2/8.7)

5/4

COPD

n = 7

5.6 ± 1.7 (4.2/8.5)

2/7

Controls

n = 9

7.4 ± 2.1 (4.2/9.1)

6/3

RLT

n = 13

8.6 ± 0.4 (7.6/9.1)

13/0

HOPE

n = 12

4.3 ± 0.6 (3.2/5.1)

0/12

  1. RIN RNA integrity number, RLT preservation by storage in RLT buffer, HOPE preservation via HOPE-fixation technique