Fig. 2

H19 knockout inhibited bleomycin-induced pulmonary inflammation in mice. a Representative immunofluorescence (IF) images of CD45 positive cells in lungs of Sham Wt (n = 10), Sham H19^−/− (n = 7), Wt BLM (n = 9) and H19^−/− BLM (n = 9) mice. b The relative mRNA levels of the inflammatory marker genes including F4/80, CD11b, Ccl20, IL1b and Ym1, in the lungs of Sham Wt (n = 10), Sham H19^−/− (n = 7), Wt BLM (n = 9) and H19^−/− BLM (n = 9) mice. The Hprt1 was used as an internal control. c Representative images of the immune blots of Il6, p-Stat3, Stat3 and β-Actin. d Relative protein expression levels of Il6 and p-Stat3 were normalized using β-actin or Stat3. Statistical significance: *p < 0.05; **p < 0.01; ns not significant