Fig. 1

H19 increased in lungs of pulmonary fibrosis. a Data for H19 mRNA expression extracted from the GEO database, comparing whole extracts of lung tissue from human patients diagnosed with IPF (n = 50) and other interstitial lung diseases (ILD) (n = 35) and from control subjects (n = 55) (GSE47460). b Data for H19 mRNA expression extracted from the GEO microarray database, comparing whole extracts of lung tissue from bleomycin-induced lung fibrosis in rat (n = 5 animals per bleomycin-treated group and n = 22 for saline controls). c Representative images of FISH targeting H19 (red) and immunofluorescence (IF) staining of Sftpc in lungs of Sham and bleomycin-treated mice. d The relative H19 mRNA levels in the lungs of Sham wild type (Wt, n = 8), Sham H19 knockout (H19^−/−, n = 6) Wt bleomycin (BLM, n = 8) and H19^−/− BLM (n = 10) mice were determined by real-time RT-PCR. The Hprt1 was used as an internal control. e Representative images of the DNA agarose gels of H19 and Hprt1