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Fig. 2 | Respiratory Research

Fig. 2

From: Dexamethasone rescues TGF-β1-mediated β2-adrenergic receptor dysfunction and attenuates phosphodiesterase 4D expression in human airway smooth muscle cells

Fig. 2

Overnight TGF-β1 treatment impairs CTX-induced MLC dephosphorylation in HASM. Phosphorylation of myosin light chain (pMLC) following Cch (20 μM, 13 min) or TGF-β1 (10 ng/ml, 18 h) or was assessed following ISO (1 μM, 10 min), FSK (10 μM, 15 min), or CTX (0.25 μg/ml, 45 min) treatment. SB-431542 (5 μM, 1 h prior to TGF-β1 treatment), a TGF-β1 receptor inhibitor, was used as a control. All treatments (ISO/FSK/SB/CTX) significantly attenuated TGF-β1-induced pMLC (p < 0.05). Phosphorylation of MLC was normalized to total MLC for each experiment. *p < 0.05 as assessed by one-way ANOVA, and comparisons between two conditions assessed by Student’s t-test. Data is representative of n = 5–6 distinct HASM cell lines

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Respiratory Research

ISSN: 1465-993X

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