Fig. 4

ECM remodeling in lung tissue after BTT. A) ECM regulation and collagen amount in lung tissue post-BTT in ND and HFD mice. Sirius Red staining of lung tissue sections. Arrowhead = fibrosis, arrow = damaged lung tissue with blood cell infiltration. Pictures were taken with an Olympus IX81 using Xcellence v.1.2. Scale = 50 μm at 40x magnification or 200 μm at 10x magnification. B) Quantification of collagen in the lung using a chemical collagen quantification assay kit. Results are presented as mean ± SEM. C) Schematic presentation of Sirius Red stained whole lung tissue sections. Abbreviations: HFD, high fat diet; ND, normal diet