Fig. 8

Nrf2 deficiency aggravated ALI and ferroptosis in mice induced by seawater drowning. Images (a) and quantification (b) of Nrf2 expression levels in lung were analyzed by Western blot (n = 3). (c) Immunofluorescence images were used to detect the expression of Nrf2 (red) in mouse lung tissue. Nuclei were counterstained with DAPI (blue). Original magnification × 200. Bar = 50 μm. (d) Lung wet/dry weight ratio (n = 4). (f) Gross morphology of mice lung. H&E stained pathological tissue images (g) and lung injury scores (e) (n = 4). Original magnification × 200 and × 400. Scale bars: 50 μm. (h) Micro CT images of mouse lungs. (i) Lung volume quantification was analyzed by Analyze 12.0 software based on the Micro CT data (n = 4). Kit detected GSH (j) and MDA (k) contents in mouse lung tissue (n = 4). (l) qPCR was used to detect the expression of ferroptosis-related gene Ptgs2 mRNA (n = 4). Data were presented as the mean ± SD. ^⁎P < 0.05 vs. Control group, ^#P < 0.05 vs. SW group