Fig. 7

Nrf2 agonist DMF inhibited ferroptosis and improved ALI in mice induced by seawater drowning. Images (a) and quantification (b) of Nrf2 expression level in lung were analyzed by Western blot (n = 3). (c) Immunofluorescence images were used to detect the expression of Nrf2 (red) in mouse lung tissue (n = 3). Nuclei were counterstained with DAPI (blue). Original magnification × 200. Bar = 50 μm. (d) Lung wet/dry weight ratio (n = 4). (f) Gross morphology of mice lung. H&E stained pathological tissue images (g) and lung injury scores (e) (n = 4). Original magnification × 200 and × 400. Bar = 50 μm. (h) Micro CT images of mouse lungs. (i) Lung volume quantification was analyzed by Analyze 12.0 software based on the Micro CT data (n = 3). Kit detected GSH (j) and MDA (k) contents in mouse lung tissue (n = 4). (l) qPCR was used to detect the expression of ferroptosis-related gene Ptgs2 mRNA (n = 4). Data were presented as the mean ± SD. ^⁎P < 0.05 vs. Control group, ^#P < 0.05 vs. SW group