Fig. 5

Nrf2 protected mitochondria and regulated ferroptosis related gene expression. (a) Fluorescence images of 5 μM JC-1 stained MLE-12 cells. Scale bars: 50 μm. The ratio of red and green fluorescence reflected changes of mitochondrial membrane potential (n = 3). Fluorescence images (b) and relative quantification (d) of MitoSox Red (5 μM) stained MLE-12 cells. Original magnification × 200. Bar = 50 μm. (c) The mitochondrial membrane potential stained with rhodamine 123 (5 μM) was detected by a microplate reader (n = 4). Relative mRNA expression of ferroptosis-related genes GPX4 (e), FTH1 (f) and Ptgs2 (g) in MLE-12 cells (n = 4). (H) The protein–protein interaction (PPI) network of Nrf2, GPX4, Ptgs2 and FTH1 (String). Data were presented as the mean ± SD. ^⁎P < 0.05 vs. Control group, ^#P < 0.05 vs. SW group