Fig. 4
From: Nrf2 protects against seawater drowning-induced acute lung injury via inhibiting ferroptosis
Nrf2 alleviated GSH depletion, lipid peroxidation and accumulation of ROS induced by seawater stimulation in MLE-12 cells. The content of GSH (a), MDA (b) and SOD (c) in MLE-12 cells (n = 4). (d) Intracellular ROS was monitored using the DCFH-DA fluorescent probes (n = 4). (e) Images of intracellular ROS levels in MLE-12 cells stained by DHE (10 μM) fluorescent probes. Original magnification × 200. Bar = 50 μm. (f) lipid ROS was analyzed using 2 μM BODIPY® 581/591 C11 with flow cytometry (n = 3). (g) Quantitative results of lipid ROS (n = 3). Data were presented as the mean ± SD. ⁎P < 0.05 vs. Control group, #P < 0.05 vs. SW group