Fig. 2

Inhibition of ferroptosis alleviated seawater-induced MLE-12 cell damage. (a) and (b). CCK-8 assay for the determination of cell viability (n = 4). (d) Cell death was analyzed using Annexin V-FITC/PI staining with flow cytometry (n = 3). (c) Quantitative results of the percentage of cell death (n = 3). (e) Intracellular ROS was monitored using the DCFH-DA fluorescent probes (n = 4). (f) Images of intracellular ROS levels in MLE-12 cells stained by DHE (10 μM) fluorescent probes. Scale bars: 50 μm. (g) lipid ROS was analyzed using 2 μM BODIPY® 581/591 C11 with flow cytometry (n = 3). (h) Quantitative results of lipid ROS (n = 3). Kit detected GSH (i), MDA (j) and SOD (k) levels in MLE-12 cells (n = 4). Data were presented as the mean ± SD. ^⁎P < 0.05 vs. Control group, ^#P < 0.05 vs. SW group