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Fig. 1 | Respiratory Research

Fig. 1

From: Nrf2 protects against seawater drowning-induced acute lung injury via inhibiting ferroptosis

Fig. 1

Seawater exposure induced MLE-12 cell damage. MLE-12 cells were exposed to 25% seawater for different times (a-d). (a) Cell morphology images (Original magnification × 200. Bar = 50 μm). (b) CCK-8 assay for the determination of cell viability (n = 4). (c) Cell death was analyzed using Annexin V-FITC/PI staining with flow cytometry (n=3). (d) Quantitative results of the percentage of cell death (n = 3). (e) The expression of Ptgs2 mRNA in MLE-12 cells was measured by qPCR after 6 h of seawater exposure (n = 4). The level of Nrf2 expression was determined by Western blot (f) and qPCR (g) (n = 3). Data were presented as the mean ± SD. P < 0.05 vs. Control group

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