Fig. 1

Effects of rPTX3 treatment on bronchoalveolar lavage fluid (BALF). a Timeline of the protocol for establishing neutrophilic and eosinophilic asthma model. In general, mice were sensitized in the presence of either OVA/LPS or OVA/Alum, followed by exposure to OVA aerosols for 5 consecutive days. One hour before challenge, mice were administrated intranasally with rPTX3. One day later, BALF was isolated to determine differential cell counts and cytokines levels. b Representative cytospin images of Liu’s solution staining. c Total cells, d neutrophils, e eosinophils, f lymphocytes, and g macrophages were counted in BALF. Data are shown as the mean ± SEM (n = 6–10), *P < 0.05, **P < 0.01, and ***P < 0.001. Data were analysed using one-way analysis of variance (ANOVA) followed by the Tukey-Kramer post hoc test for multiple comparisons