Fig. 7

MiR-124 inhibits tumor growth by targeting AKT2 in vivo. a and b 2 × 10⁶ A549 cells were injected into nude mice (n = 2 mice per group). After tumor formation, miR-124 agomir and miR-NC agomir were injected into each tumor. Thirty-six days later, mice were euthanasia and tumors were surgically resected. Representative images of mice (a) and tumors (b) are shown. c The xenograft tumor volumes were measured every 2–3 days for total 36 days. Graph of tumor growth curves at the experimental endpoint. d Quantification of tumor weights from control and miR-124 agomir group. e and f qRT-PCR analysis of miR-124 and AKT2 mRNA expression in each excised tumor. U6 and ACTB were used as internal controls, respectively. g Representative images of H&E staining and IHC staining show tumor cells and the expression of AKT2 and KI67 in excised tumors from control or miR-124 agomir group. Scale bar, 200 um. *P < 0.05 **P < 0.01 ***P < 0.001