Fig. 5

Depletion of AKT2 inhibits cell migration and invasion in LUAD cells. a AKT2-silenced A549 and H1299 cells were allowed to migrate through an 8-μM pore in Transwells. The migratory cells were stained and counted in at least three microscopic fields. Cells were then treated as above and allowed to invade through the matrigel-coated membrane in the transwell inserts. Invaded cells were stained and counted under a microscope. Representative images (left) and the migratory cell numbers (right) were shown. Scale bar, 2 mm. b The mRNA expression of CDH1, CDH2, VIM, SNAI1, SNAI2, MMP7, MMP9, ZEB1 and ZEB2 were detected by qRT-PCR in AKT2 silenced A549 (upper) and H1299 (bottom) cells. c The expression of N-cadherin, Vimentin, Slug, MMP2, MMP7, MMP9, AKT, p-AKT, Erk and p-Erk protein levels were examined by western blot in AKT2 silenced A549 (left) and H1299 (right) cells. Each experiment was performed in triplicate independently and values are expressed as mean ± SD. *P < 0.05 **P < 0.01 ***P < 0.001