Fig. 4
From: AKT2 drives cancer progression and is negatively modulated by miR-124 in human lung adenocarcinoma
Silencing AKT2 attenuates the cell cycle progression of LUAD cells without affecting apoptosis. a Flow cytometry cell cycle analysis was performed in A549 and H1299 cells transfected with Si-NC, Si-AKT2–1 and Si-AKT2–3. The distribution (%) of G0/G1, S and G2/M phases are shown in the histograms (bottom). b Flow cytometry apoptosis assay was performed in A549 and H1299 cells transfected with Si-NC, Si-AKT2–1 and Si-AKT2–3. 48 h post-transfection, cells were harvested and stained with Annexin V/FITC and propidium iodide (PI). The percentage of apoptotic cells are shown in the right panel. The date values represent mean ± SD of three measurements independently. NS: no significance. *P < 0.05; **P < 0.01; ***P < 0.001