Fig. 3

Knockdown of AKT2 significantly prevents cell proliferation in LUAD cell lines. a and b AKT2 levels were detected by western blot (a) and qRT-PCR (b) in multiple NSCLC cell lines. c and d A549 and H1299 cells were transfected with control siRNA or siRNA against AKT2, the effective knock down of AKT2 expression was validated by western blot (c) and qRT-PCR (d). e and f CCK-8 assay was performed to detect the cell growth in AKT2 silenced A549 (e) and H1299 (f) cells. g Colony formation abilities were detected in AKT2 silenced A549 and H1299 cells. Representative images of colonies for cell proliferation were shown (left). The colony numbers in AKT2 silenced cells were normalized to negative control (right). h An EdU staining assay was performed to determine the proliferation ability of AKT2 silenced A549 and H1299 cells (left). EdU-positive ratio were shown (right). Scale bar, 2 mm. Each experiment was performed in triplicate independently. Student’s t-test was used for statistical analysis and data are presented as the mean ± SD.*P < 0.05; **P < 0.01; ***P < 0.001