Fig. 1

AKT2 is overexpressed in human NSCLC tissues. (a and c) Relative quantification of AKT2 expression was analyzed by qRT-PCR assay in 45 NSCLC tissues (T) and adjacent noncancerous tissues (N). ACTB mRNA levels were used as internal control for normalization of AKT2 mRNA expression. b Western blot analysis was performed to detect the AKT2 protein expression in 8 selected NSCLC tissues and adjacent tissues (left) and the quantification of relative AKT2 protein levels were shown in histogram (right). β-actin was used as internal control. d-f The public datasets from GEO (GSE 10072, GSE 31210 and GSE 32863) were used to verify AKT2 mRNA levels in NSCLC. g The public data from TIMER database was used to detect AKT2 mRNA expression in both LUAD tissues and LUSC tissues. h Tissue and normal samples from randomly selected patients with NSCLC were either stained with Hematoxylin and eosin (HE) (upper, n = 2 per group) or immunohistochemically (IHC) stained with an AKT2 antibody (bottom, n = 2 per group). Scale bar, 200 um. T: NSCLC tumor tissues N: Adjacent normal tissues. *P < 0.05; **P < 0.01; ***P < 0.001