Fig. 5
From: Sonic hedgehog signalling as a potential endobronchial biomarker in COPD
Shh activating ligand is deficient in COPD bronchi. a. Representative micrograph showing a ROI of a bronchial brushing stained for basal cells (pancytokeratin, KP, white); Gli2 (green); Ptch1 (red, left panel); Hhip (red, right panel); and cell nuclei (DAPI, blue). Magnification corresponding to the selected area is shown. b. Representative micrograph showing a ROI of a bronchial biopsy stained for cilia (Acetylated tubulin or Arl13b, green); Ptch1 (red, left panel); Hhip (red, right panel); and cell nuclei (DAPI, blue). Magnification corresponding to the selected area is shown. c. Representative micrograph showing a ROI of a bronchial biopsy stained for cilia (Arl13b, green); Shh (red) and cell nuclei (DAPI, blue). Magnification corresponding to the selected area is shown. d. Dot plot with median representing Shh concentrations measured by ELISA in bronchial BALF from non-COPD (n = 15) and COPD patients (n = 15). **, p < 0.001