Fig. 4
From: Sonic hedgehog signalling as a potential endobronchial biomarker in COPD
Gli2 transcription factor is decreased in whole bronchial epithelium from COPD patients. a. Representative micrograph showing a ROI of a bronchial biopsy stained for cilia (Acetylated tubulin, green); Gli2 (Gli2, red); basal cells (p63, white) and cell nuclei (DAPI, blue) in both non-COPD (upper panel) and COPD patients (lower panel). Magnification corresponding to the selected area is shown. Insets depict localization of the Gli2 transcription factor. b. Dot plot with median showing the intensity of Gli2 mean grey value (Arbitrary units, AU) in whole bronchial epithelium in non-COPD (n = 12) and COPD patients (n = 19). **, p < 0.001 C. Linear regression of the intensity of Gli2 mean grey value according to FEV1 (% predicted) in non-COPD (n = 12) and COPD patients (n = 19). Non-COPD patients are represented by black circles and COPD patients are represented by red circles