Fig. 6

Effect of FGL1 knockdown on PC9 cells. a Relative expression of FGL1 protein in PC9 cells after FGL1 knockdown as measured by western blotting. b Relative expression of FGL1 mRNA in PC9 cells after FGL1 knockdown as measured by RT-qPCR (x ± s, n = 3). c Proliferation of PC9 cells inhibited by gefitinib after FGL1 knockdown as measured by CCK-8 assy. d Comparison of IC50 values of gefitinib in PC9 cells before and after FGL1 interference. e Colony-forming potential assessed in PC9 cells and PC9 cells. f Apoptotic rate of PC9 cells as evaluated by flow cytometry. g Western blot analysis of the effect of FGL1 on EGFR, p-EGFR, caspase 3 and PARP1 protein in the sensitive NSCLC cell line PC9. Control: blank control group; NC-siRNA: mice transfected with NC-siRNA; Gefitinib: mice treated with gefitinib alone; FGL1-siRNA: mice treated with FGL1-siRNA-transfected cells; gefitinib+FGL1-siRNA: mice transfected with FGL1-siRNA cells and treated with gefitinib. *P < 0.05 compared with control group; **P < 0.01 compared with control group; #P < 0.05 compared with gefitinib group; △P < 0.01 compared with FGL1-siRNA group; &P < 0.01 compared PC9 cells