Functional glutamate transporters are expressed in the carotid chemoreceptor

Respiratory Research

Table 1 Sequence of human primers used in RT-PCR experiment. VGluT: vesicular glutamate transporter, EAAT: excitatory amino acid transporter, Tm: temperature, F: forward primer, R: reverse primer.

Gene	Accession	Primer	PCR Cycle no.	Annealing Tm (⁰C)
VGluT1	NM_020309.4	F: 5’-TGCCTCTCAGGGGTAGTGAA-3’	45	53.5
VGluT1	NM_020309.4	R: 5’-ATCAATCCCTGGAATGGCGG-3’	45	53.5
VGluT2	NM_020346.2	F: 5’-GGGAGACAATCGAGCTGACG-3’	45	56.5
VGluT2	NM_020346.2	R: 5’-TGCAGCGGATACCGAAGGA-3’	45	56.5
VGluT3	NM_139319.3	F: 5’-CTCAGAGGTGCCCCTCATTC-3’	45	53.5
VGluT3	NM_139319.3	R: 5’-TGCATAAACCGGCAAAGATGTG-3’	45	53.5
EAAT1	NM_004172.5	F: 5’- AGCAGGGAGTCCGTAAACG-3’	35	51.8
EAAT1	NM_004172.5	R: 5’-AGCATTCCGAAACAGGTAACTTT-3’	35	51.8
EAAT2	NM_004171.4	F: 5’-CCTGACGGTGTTTGGTGTCAT-3’	35	54.4
EAAT2	NM_004171.4	R: 5’-CAAGCGGCCACTAGCCTTAG-3’	35	54.4
EAAT3	NM_004170.5	F: 5’-TTTCTGTACCACTCTCATTGCTG-3’	35	51.8
EAAT3	NM_004170.5	R: 5’-TCCACCGTACTGACTTCAGGG-3’	35	51.8
GAPDH	NM_002046.7	F: 5’-GCAGGGGGGAGCCAAAAGGGT-3’	40	53
GAPDH	NM_002046.7	R: 5’-TGGGTGGCAGTGATGGCATGG-3’	40	53

ISSN: 1465-993X