Table 4 Summary of CXCR4 panel multiplex immunohistochemistry analysis

Percentage “cells” = Hoechst+

30.69

13.6

28.91

11.5

0.209

Single CXCR4+

0.23

0.2

0.80

0.5

0.007**

1.494

Single E-Cadherin+

10.59

10.5

5.20

3.9

0.318

Single CXCL12+

9.64

5.9

7.32

5.8

0.456

Double CXCR4 + E-Cadherin+

0.08

0.1

0.38

0.2

0.007**

1.853

Double CXCR4 + CXCL12+

0.15

0.2

0.38

0.1

0.049*

1.349

Double E-Cadherin+CXCL12+

4.67

3.5

3.24

2.4

0.620

Triple CXCR4+ E-Cadherin+ CXCL12+

0.07

0.1

0.31

0.2

0.011*

1.815

Triple CXCR4+ E-Cadherin- CXCL12-

0.07

0.1

0.34

0.1

0.097

Triple CXCR4+ E-Cadherin+ CXCL12-

0.01

0.0

0.07

0.1

0.073

Triple CXCR4+ E-Cadherin- CXCL12+

0.09

0.2

0.07

0.2

0.383

Triple CXCR4- E-Cadherin- CXCL12-

15.06

8.3

19.30

8.9

0.535

Triple CXCR4- ECadherin- CXCL12+

4.88

4.9

4.00

5.1

0.535

Triple CXCR4- E-Cadherin+ CXCL12+

4.61

3.4

2.93

3.4

0.535

Triple CXCR4- E-Cadherin+ CXCL12-

5.90

7.8

1.88

7.4

0.456

CD45 Panel

Mann-Whitney U

Effect Size

Mean1

SD

Mean2

SD

p-value

Percentage “cells” = Hoechst+

28.13

14.6

24.57

10.9

0.456

Single CXCR4+

0.62

0.8

3.88

3.2

0.026*

1.378

Single CD45+

18.83

9.2

12.62

9.4

0.209

Single CXCL12+

6.40

3.8

6.15

5.1

0.710

Double CXCR4 + CD45+

0.17

0.1

0.78

0.8

0.026*

1.041

Double CXCR4 + CXCL12+

0.22

0.3

1.61

1.6

0.011*

1.186

Double CD45 + CXCL12+

5.28

3.0

3.49

3.9

0.209

Triple CXCR4 + CD45 + CXCL12+

0.06

0.1

0.30

0.3

0.128

Triple CXCR4+ CD45- CXCL12-

0.30

0.5

1.78

1.8

0.128

Triple CXCR4+ CD45+ CXCL12-

0.11

0.1

0.48

0.2

0.097

Triple CXCR4+ CD45- CXCL12+

0.16

0.2

1.32

0.7

0.011*

2.245

Triple CXCR4- CD45- CXCL12-

7.88

6.4

7.52

4.6

0.902

Triple CXCR4- CD45- CXCL12+

0.96

0.9

1.34

0.9

> 0.9999

Triple CXCR4- CD45+ CXCL12+

5.22

2.9

3.20

3.4

0.209

Triple CXCR4- CD45+ CXCL12-

13.45

9.2

8.64

8.1

0.318