Fig. 6
From: CXCR4+ cells are increased in lung tissue of patients with idiopathic pulmonary fibrosis
Brightfield immunohistochemistry of lung tissue from patients with end-stage fibrotic interstitial lung disease (ILD) and normal donors. Lung tissue was sampled from 10 patients with idiopathic pulmonary fibrosis (IPF), 8 patients with non-IPF ILD (2 non-specific interstitial pneumonia, 4 hypersensitivity pneumonitis, 2 connective tissue disease associated ILD and 10 non-diseased control (NDC) normal donors. Brightfield immunohistochemistry was used to examine CXCR4 expression in lung tissue with cell nuclei counterstained with Mayer’s hematoxylin. a Minimal CXCR4 expression is seen in NDC lung tissue. Strong CXCR4 staining is seen in tissue taken from lung apices and bases in both IPF and non-IPF ILD patients. Scale bar 200 μm (b) CXCR4 expression was semi-quantified in 60 samples of lung tissue from lung apex (∆) and lung bases (•). CXCR4 is increased in IPF and non-IPF ILD compared to NDC donors. c Higher magnification of base-derived lung tissue shown in (A) demonstrates CXCR4 expression in distal airways localised to epithelium in IPF and non-IPF ILD. Scale bar 200 μm