Fig. 3

Various phenotypes of CXCR4⁺ cells are observed in lung tissue. Representative images of lung tissue from 3 patients with IPF and 3 non-diseased control (NDC) donors. Tissue was stained simultaneously for CXCR4 (green), CXCL12 (red), and either e-cadherin or CD45 (yellow) to identify epithelial cells and myeloid cells respectively. Cell nuclei was counterstained with Hoechst dye. Eight cell phenotypes were observed in NDC (a-c, g-i) and IPF (d-f, j-l) tissue. CXCR4⁺ (↑), e-cadherin⁺ (#), CD45⁺ (>), CXCL12⁺ (*), CXCR4⁺/E-Cadherin⁺ (◊), CXCR4⁺/E-Cadherin⁺/CXCL12⁺ (◆), CXCR4⁺/CD45⁺ (∆) and CXCR4⁺/CD45⁺/CXCL12⁺ (‡). Scale bar 50 μm