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Fig. 2 | Respiratory Research

Fig. 2

From: CXCR4+ cells are increased in lung tissue of patients with idiopathic pulmonary fibrosis

Fig. 2

Two populations of CXCR4+ cells are seen in peripheral lung tissue. Multiplex immunohistochemistry was used to identify two populations of CXCR4-expressing cells in lung tissue from 7 patients with IPF and 7 non-diseased control (NDC) donors. Tissue was stained simultaneously for CXCR4 (green), CXCL12 (red), and either e-cadherin or CD45 (yellow) to identify epithelial cells and myeloid cells respectively. Cell nuclei was counterstained with Hoechst dye. In the e-cadherin panel (a-d) CXCR4+/e-cadherin+ cells can be seen in areas of honeycombing (↑) that may or may not also express CXCL12. In NDC tissue (a-b) the occasional circulating cell can be seen in the alveolar space (◊) but in IPF tissue (c-d) this has been replaced by dense fibrosis with fibroblastic foci (‡). In the CD45 panel (e-h) CXCR4+ cells can be seen surrounding the airway lumen (♦) in IPF tissue but there is a lack of CXCR4+ cells in blood vessels (∆). Scale bar 200 μm

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