Fig. 3
From: The SPLUNC1-βENaC complex prevents Burkholderia cenocepacia invasion in normal airway epithelia
The plasma membrane SPLUNC1-βENaC complex protects cells from B. cenocepacia invasion. αβγENaC-transfected HEK293T cells were pre-treated with rSPLUNC1 or vehicle for 1 h, followed by infection J2315 labeled with Syto9 for 1 h at an MOI of 30. Representative XZ (a) scanned fluorescence images of B. cenocepacia-Syto9 (green) and HEK293T labeled with SNARF-1 (red) (N = 3). b Graph of mean pixel intensity of J2315 in relationship to cell. Cells pre-treated with vehicle (black) or with rSPLUNC1 (blue) and curve fitted using Lorentizian (N = 36), p = 0.0001. c-d SPLUNC1 was co-expressed with different combinations of ENaC subunits and infected with J2315 for 2 h at MOI of 30 (N = 4). Relative J2315 burden in the (c) media and (d) lysate. * p < 0.05, ** p < 0.01, **** p < 0.0001. Scale bars are 20 μm