Fig. 6

Overexpression of A Disintegrin and A Metalloproteinase Domain 15 (ADAM15) in human THP-1 cells reduces cigarette smoke extract (CSE)-induced release of cytokines and proteinases. In a, ADAM15 steady state mRNA levels were measured in total cellular RNA samples isolated from differentiated THP-1 cells 24 h after they were electroporated with the pcDNA3.1-ADAM15 plasmid (ADAM15 over-expression [OE] vector) or a control vector (Vector), as described in Methods. In b-e, THP-1 cells were electroporated with either the ADAM15 OE or the control vector and then incubated with 5% CSE for the time indicated. Levels of MMP-9 (b), MMP-12 (C), CCL-2 (d) and TNF-α (e) protein secreted into the media were quantified using commercial ELISA kits. Data are mean + SD. Data were analyzed using a One-Way ANOVA followed by pair-wise testing with two-tailed Student’s t-tests. *, P < 0.05 versus the group indicated