Skip to main content
Fig. 1 | Respiratory Research

Fig. 1

From: ADAM15 expression is increased in lung CD8+ T cells, macrophages, and bronchial epithelial cells in patients with COPD and is inversely related to airflow obstruction

Fig. 1

A Disintegrin and A Metalloproteinase Domain 15 (ADAM15) expression is increased in lung samples and alveolar macrophages (AMs) from patients with chronic obstructive pulmonary disease (COPD) versus controls. In a, ADAM15 steady state mRNA levels were measured in lung samples from non-smokers, smokers, and patients with COPD with GOLD stage I-II and GOLD stage III-IV disease using real time RT-PCR (n = 17–31 subjects/group). Data are mean + SD. Data were analyzed using one-way ANOVA followed by pair-wise testing with 2 tailed Student’s t-tests. *, P < 0.001 versus non-smoker group or the group indicated. In b, ADAM15 steady state mRNA levels were measured in AMs from non-smokers, smokers, and patients with COPD (all had GOLD stage I-III disease and 79% of these patients had GOLD stage I or II disease) in the BAL cohort using real time RT-PCR (n = 6–15 subjects/group). Data are mean + SD. Data were analyzed using one-way ANOVA followed by pair-wise testing with 2 tailed Student’s t-tests. *, P < 0.001 versus non-smoker control or the group indicated. In c-d, ADAM15 and a housekeeping control (heat shock protein 90; HSP90) were quantified in AMs from non-smokers, smokers, and patients with COPD using Western blotting and densitometry. The ADAM15 levels were normalized to HSP90 levels measured in the samples and expressed as a % of the values for the non-smoker control group. The images shown in c are representative of 6–9 subjects/group. d: Data are mean + SD (n = 6–9 subjects/group). Data were analyzed using a One-Way ANOVA followed by pair-wise testing with 2 tailed Student’s t-tests. *, P < 0.001 versus non-smoker control or the group indicated

Back to article page