Fig. 6

Nebulized miR-138-5p restores the expression of KCNK3 and SLC45A3 in the lungs.a Diagram of the miR-138-5p putative binding site in the human KCNK3 3′ UTR (B) mRNA expression of KCNK3 in the lungs from the control rats, MCT- and anti-miRNA-control-treated rats and MCT- and anti-miR-138-5p-treated rats (n = 5 different rats per condition). b Network analysis using Ingenuity Pathway Analysis (IPA) software, with the following settings: confidence levels: experimentally observed; species: human, rat. This figure shows that miR-138-5p was shown to regulate the expression of several genes, including SLC45A3 (solute carrier family 45 member 3), ALDH1A2 (aldehyde dehydrogenase 1 family member A2), ROCK2 Rho-associated coiled-coil containing protein kinase 2), TERT (telomerase reverse transcriptase), RHOC (Ras homologue family member C), VCAN (versican) (c) SLC45A3, ALDH1A2 and ROCK2 lung protein expression analysed by western blot (n = 5 per group). Quantification of lung SLC45A3, ALDH1A2 and ROCK2 protein expression. (n = 6 different rats per condition). *P < 0.05, **P < 0.01, ***P < 0.001