Fig. 5
In vivo inhibition of miR-138-5p reduces lung inflammation and inflammatory cell infiltration in MCT-PH rats.a CD45 (a pan-leucocyte marker) protein expression in the lungs was analysed by western blot analysis (n = 6 per group). Quantification of the CD45 protein expression in the lungs. (n = 6 different rats per condition). b Representative images of T lymphocyte (CD3-positive cells) accumulation and quantification of the mean number of T lymphocytes (CD3+ cells) per mm2 in the lung sections from the Control, MCT + anti-miR-Control and MCT + anti-miR138–5p groups (n = 6 different rats per condition). *P < 0.05, **P < 0.01, ***P < 0.001. c Representative images of macrophage (CD68-positive cells) accumulation and quantification of the mean number of macrophages (CD68+ cells) per mm2 in the lung sections from the Control, MCT + anti-miR-Control and MCT + anti-miR138–5p groups (n = 6 different rats per condition). *P < 0.05, **P < 0.01, ***P < 0.001