Fig. 3

In vivo inhibition of miR-138-5p reduces pulmonary vascular remodelling in rats with MCT-PH.a Representative haematein and eosin staining of the paraffin-embedded lung sections from the control, MCT + anti-miR-control, and MCT + anti-miR-138-5p groups. Scale bar = 100 μm (B) Vascular wall area (%) in the control, MCT + anti-miR-control, and MCT + anti-miR-138-5p groups (n = 5 different rats per condition). b Quantification of the percentage of nonmuscularized vessels (VWF-positive vessels, left panel), muscularized vessels (αSMA- and VWF-positive vessels, middle panel) and occluded vessels (right panel) (n = 6 different rats per condition). c Representative images of pulmonary arteries by Orcein staining (two elastic fibres) and quantification of the mean smooth muscle thickness (μm) of the pulmonary arteries from the Control, MCT + anti-miR-Control and MCT + anti-miR138–5p groups (n = 6 different rats per condition). *P < 0.05, **P < 0.01, ***P < 0.001