Fig. 2
From: CDK4/6 inhibition enhances pulmonary inflammatory infiltration in bleomycin-induced lung fibrosis
Palbociclib promotes lung inflammation following bleomycin challenge. a Total cell count in the bronchoalveolar lavage (BAL) of control mice (Saline) and mice with bleomycin-induced lung injury with (Bleo+PD) and without palbociclib (Bleo) treatment. Samples were collected 14 days after intratracheal bleomycin or saline instillation. The lung was lavaged with 1 ml of PBS supplemented with protease inhibitors. b Heatmap representation of the relative proportion of inflammatory cell populations in the BAL. z-scores are shown. c Absolute numbers of inflammatory cells in the BAL fluid of control and bleomycin-treated mice with and without palbociclib. MoAM: monocyte-derived macrophages; gdTCR: γδ T-cells. d Protein levels of neutrophil elastase (Elane) were determined in cell-free bronchoalveolar lavage fluid. e Activity of myeloid peroxidase (MPO) was measured by reduction of hydrogen peroxide using o-dianisidine as hydrogen donor and determining optical density at 450 nm (n = 2–6)