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Fig. 1 | Respiratory Research

Fig. 1

From: CDK4/6 inhibition enhances pulmonary inflammatory infiltration in bleomycin-induced lung fibrosis

Fig. 1

Palbociclib decreases collagen deposition but does not improve lung function in the bleomycin-mouse model. a Schematic representation of palbociclib treatment in bleomycin-induced lung fibrosis. Lung injury was induced by intratracheal bleomycin (Bleo) instillation (0.8 units/g bodyweight) at day 0, followed by daily oral gavage with 150 mg/kg bodyweight palbociclib (PD 0332991) in a subgroup of mice (Bleo+PD), starting from day 1. Lung function measurements and organ collection were performed 14 days post bleomycin. Control animals received intratracheal saline. b Lung function measurements were performed using a flexiVent FX1 (Scireq) system. FVC: forced vital capacity, FEV0.1: forced expiratory volume after 0.1 s; Kruskal Wallis test; ** p < 0.01. Collagen content of the lung was measured on Sirius Red stained lung sections of the entire left lung lobe (c) with semi-automated quantification (d) using the Visiopharm integrated software. e Hydroxyproline measuements were performed on tissue homogenates from right lung pieces. f Representative immunoblot of collagen I in lung homogenates of saline, bleomycin, and bleomacin+palbociclib treated mice. α-tubulin served as a loading control. g Bodyweight curves of mice following bleomycin-induced lung injury. n = 4–6, data are shown as mean ± SEM. Bleo and Bleo+PD groups were compared by two-way ANOVA with Bonferroni post-test; *p < 0.05, **p < 0.01, ***p < 0.001

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