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Fig. 4 | Respiratory Research

Fig. 4

From: Host DNA released by NETosis in neutrophils exposed to seasonal H1N1 and highly pathogenic H5N1 influenza viruses

Fig. 4

Neutrophil extracellular trap formation upon influenza virus infection with and without pre-treatment of virus-free condition media. a SYTOX green staining for the extracellular DNA in [1] A/VN/1203/04 (H5N1), [2] A/HK/483/97 (H5N1), [3] A/OK/447/08 (H1N1) and [4] A/HK/415742/09 (H1N1pdm) infected neutrophil at 6 hpi. The A/OK/447/08 (H1N1) infected neutrophil showed the entrenched morphology of the staining represents stretching out of DNA material from the nucleus of neutrophil which is one of the typical indicators of NET formation. b Scanning electron microscopy of [1] mock, [2] A/HK/483/97 (H5N1) [3] A/OK/447/08 (H1N1) infected neutrophils at 6 hpi, scale bar = 50 μm

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