Fig. 1

Neutrophil transepithelial migration in a primary human type I-like pneumocyte transwell system. a Schematic diagram showing the setup of transepithelial migration. Pneumocytes (Pϕ), indicated as the blue cells, were seeded at the basolateral side of the transwell membrane with a pore size of 3.0 μm. Naïve neutrophils (Nϕ) were added at the apical chamber, as if they infiltrate from the basolateral size of Pϕ to the alveolar space (basolateral chamber) in vivo. b Percentage of neutrophil migration across mock (grey) and influenza virus infected Pϕ layer at 6 and 24 hpi. * indicates p < 0.05 between the indicated group(s)