Fig. 6

The altered T17 EV proteome contains proteins with activating effects on neutrophil migration. a-b Differential levels of proteins involved in the pathways related to neutrophil migration identified in Table 2 that were predicted to be activated by T17-EV proteins (a) or inhibited by T2-EV proteins (b). c Peripheral blood neutrophils allowed to migrate towards; media only as negative control, EVs (30 μg/ml) from non-stimulated epithelial cells (blue) or cells stimulated with T2 (red) or T17 (green) cytokines, or the positive control (5% FBS). Each dot represents the median number of migrated cells after 3 h of incubation (2–4 replicate wells) for one donor, with group means ± SD indicated by lines. (n = 7–8) Group means were compared using mixed-effects analysis. Only adjusted p-values ≤0.05 after Tukey’s multiple comparisons test are shown (*: p ≤ 0.05, **: p ≤ 0.01)