Fig. 5

The proteome of bronchial epithelium-derived extracellular vesicles is altered upon T2 and T17 stimulation. Quantitative proteomics (tandem mass tag; TMT) was used to determine the influence of T2 and T17 cytokines on bronchial epithelium-derived extracellular vesicles. a Principle component analysis illustrating the relationship between T2-derived EVs (red), T17-derived EVs (green) and EVs isolated under non-stimulated condition (blue) (n = 3). b-c) Volcano plots of the proteome after T2 (b) and T17 (c) stimulation. Dotted lines indicate cut offs, which is 1.3 on the Y-axis (corresponding to p < 0.05) and 0.67 on the X-axis (corresponding to fold change> 1.5). d-e) Top 15 upregulated proteins in extracellular vesicles derived from bronchial epithelial cells after T2 (d) and T17 (e) stimulation based on fold change compared to non-stimulated cells f-g) Top 10 downregulated proteins in extracellular vesicles derived from bronchial epithelial cells after T2 (f) and T17 (g) stimulation based on fold change compared to non-stimulated cells