Fig. 4

Sub-chronic e-cig exposure affects mRNA expression of myeloid and innate immune response target genes analyzed by Nanostring analysis. Mice were exposed to e-cig aerosol with or without nicotine for 30 days (2 hrs/day), and sacrificed 24 hrs after final exposure. RNA was isolated from lungs and screened via nCounter Mouse Myeloid Innate Immunity Panel using Nanostring analysis. Targets were selected based on significant differences, especially (a). between) PG+Nic exposed WT and nAChRα7 KO mice, or (b) ECM remodeling focused. RNA counts were normalized to multiple housekeeping genes and quantified using nSolver. Data are shown as mean ± SEM (n=6/group; equal number of male and female mice). * P < 0.05, ** P < 0.01, *** P < 0.001 between groups; # P < 0.05, # # P < 0.01 compared with PG+Nic exposed WT group