Fig. 5

Differential abundance between upper and lower tumour lobes in salivary and lung microbiota. Each tree represents taxa with significantly different abundance relative to sample’s origin (for lung) in the upper or lower tumour lobe. For saliva, the comparison shows the significant difference in salivary microbiota between patients with tumour either in upper or lower lobe. Coloured nodes and branches represent the taxa with significantly different abundance and the intensity is proportional to log2-fold change in abundance in the favour of the lobe noted with the same colour. Statistical significance was assessed by zero-inflated general linear model using Wald’s test (DESeq), with p-value threshold of α ≤ 0.05 after BH correction. Bar chart shows the relative abundance of taxa noted in the taxonomical trees. BAL - bronchoalveolar lavage fluid, LUNG.DP - non-malignant distal piece, LUNG.PT - peritumoural tissue, LUNG. T - tumour