Fig. 4

TRIM72 promotes membrane repair of ATII-like rat lung epithelial cells (RLE). a Lentivirus-mediated TRIM72 expression (T72OE) in RLE cells. Expression of GFP marker on the L309 vector indicates infected cells; b 0.005% saponin injury releases intracellular GFP from RLE cells; c representative images of FM4–64 dye entry in CTRL and T72OE RLE cells before (0′) and after saponin treatment (40′). Scale bar = 10 μm; d quantification of FM4–64 dye entry normalized to baseline fluorescence (∆F/F0). n = 36 cells for (CTRL RLE and n = 39 for T72OE RLE cells. *P < 0.05 or **P < 0.01 compared to WT at 10′, 20′, 30′ and 40′ based on two-sided student t-tests; e Flow sorting of GFP+ primary ATII cells from lungs of the sftpc-eGFP/WT and sftpc-eGFP/TRIM72 overexpressor (T72OE) mice; f representative image of freshly sorted GFP-positive primary ATII cells; scale bar = 50 μm; g representative images of FM4–64 dye entry in primary WT and T72OE ATII cells before (0′) and after saponin treatment (20′). Scale bar = 10 μm; h quantification of FM4–64 dye entry normalized to baseline fluorescence (∆F/F0). n = 8 cells for WT ATII and n = 4 for T72OE ATII cells. *P < 0.05, or **P < 0.01 compared to WT at 5′, 10′, 15′ and 20′ based on two-tailed student t-tests